A PHOTOMETRIC NINHYDRIN METHOD FOR THE MEASUREMENT OF PROTEOLYSIS
نویسندگان
چکیده
منابع مشابه
A photometric ninhydrin method for the measurement of proteolysis.
The method, here presented, is based on the findings of Moore and Stein (1) who have shown that, over a broad range, the color produced by the interaction of an improved ninhydrin reagent with a large number of amino acids and peptides conforms with Beer’s law, although all amino acids and peptides do not produce equal color yields. This procedure has been found to be relatively simple, reasona...
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For the investigations on the chromatographic separation of amino acids outlined in the preceding communication (1)) it was necessary to have available a suitable quantitative method for the determination of the concentration of amino acids in the effluent from the column. For this purpose, the method should be sufficiently general to include the determination of most of the amino acids and pep...
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A modified ninhydrin reagent for the photometric determination of amino acids and related compounds.
The photometric ninhydrin method described previously (1) for use in the chromatography of amino acids has been the subject of further study during the development of the Dowex 50-X4 procedure (2). The adjustment of the pH of the effluent fractions prior to analysis required by the earlier method has been largely eliminated by increasing the strength of the buffer in the reagent 5-fold. A secon...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1950
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)51139-5